本品是一种经过改良的以溴酚蓝为染料，5倍浓缩的蛋白上样缓冲液。本产品中含有变性试剂，但是不含DTT、巯基乙醇等还原剂。可以用于常规的变性非还原型聚丙烯酰胺凝胶电泳的蛋白样品上样。
1.使用时，在蛋白质样品中加入1/4体积本产品，混匀；
2.将混合物用沸水浴加热3~5min；
3.待冷却后，高速离心5min，上清即可用于电泳分析。如蛋白样品在加入上样缓冲液后，无不溶性残渣，则只需短暂离心，将混合液收集到管底即可。加热后，如果管盖上出现较多冷凝水，建议轻弹离心管将冷凝水和样品混合以后，再进行高速离心步骤，此举有助于降低上样样品浓度不均的现象。
1. 为了您的安全和健康，请穿实验服并戴一次性手套操作；
2. 本产品仅限科研使用。
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